School nurses mistakenly gave the swine flu vaccine to two students who didn’t sign up for it – including a Brooklyn girl with epilepsy who wound up in the hospital.

Officials at Public School 335 in Crown Heights called an ambulance to take Nikiyah to SUNY Downstate Medical Center when she fell ill following the arm jab.

Officials admitted on Friday that a third student was mistakenly given the swine flu vaccine – an error the school nurses union blamed on the city.

Three children have received the H1N1 vaccine without parental permission – including a kid at Public School 8 on Staten Island – since the in-school program began.